| | Klub | Manager | Liga | Stadion | Sted | Stiftet |
 | Central Valley Fuego | Jermaine Jones | USL League One | Parmer Field | Fresno, California | 2020 |
| Northern Colorado Hailstorm | | USL League One | Future Legends Complex | Windsor, Colorado | 2021 |
| Dayton Dutch Lions | Matt Weston | | Miami Valley South Stadium | Dayton | 2009 |
| Saint Louis FC | Steve Trittschuh | | St. Louis Soccer Park | St. Louis | 2014 |
| Lexington SC | | USL League One | Toyota Stadium | Lexington | 2021 |
| Spokane Velocity FC | | USL League One | Skyline Sports Complex | Spokane, Washington | 2021 |
| Charlotte Eagles | | | Charlotte Christian School | Charlotte | 1991 |
| Greenville Triumph SC | | USL League One | Legacy Early College | Greenville | 2018 |
| San Diego FC | Mikey Varas | USL League One | Snapdragon Stadium | San Diego | 2023 |
| California United | | | Titan Stadium | Fullerton | 2017 |
| San Diego Loyal | | USL Championship | Torero Stadium | San Diego | 2019 |
| One Knoxville SC | | USL League One | Highmark Stadium | Knoxville, Tennessee | 2021 |
| FC Tucson | | USL League One | Kino Sports Complex | Tucson | 2010 |
| Lansing Ignite | | | Cooley Law School Stadium | Lansing | 2018 |
| Rayo OKC | | | Miller Stadium | Oklahoma City | 2015 |
| Philadelphia Union-2 | | | Goodman Stadium | Bethlehem | 2015 |
| San Francisco Deltas | | | Kezar Stadium | San Francisco | 2016 |
| Austin Aztex | | | House Park | Austin | 2008 |
| Portland Timbers 2 | Cameron Knowles | | Merlo Field | Portland | 2015 |
| Long Island Rough Riders | | | Hofstra University Soccer Stadium | South Huntington | 1994 |
| Carolina Core FC | Donovan Ricketts | MLS Next Pro | Truist Point | High Point | 2022 |
| FC Baltimore Christos | | National Premier Soccer League | Marriotts Ridge High School | Marriottsville, Maryland | 2018 |
| Peachtree City MOBA | | | MOBA Soccer Stadium | Peachtree, Georgia | 2013 |
| Club de Lyon | Héctor Almandoz | NISA | Daytona Stadium | Orlando | 2021 |
| Atlanta United 2 | Steve Cooke | USL Championship | Coolray Field | Lawrenceville | 2017 |
| Los Angeles Force | Dekel Keinan | NISA | Veterans Memorial Stadium | Long Beach | 2019 |
| Sporting KC II | Benny Feilhaber | USL Championship | Children's Mercy Park | Kansas City | 2015 |
| Milwaukee Torrent | | National Premier Soccer League | Hart Park | Milwaukee | 2015 |
| FC Miami City | | | Central Broward Park | Miami | 2014 |
| Tacoma Defiance | | USL Championship | Cheney Stadium | Seattle | 2014 |
| Virginia Cavalry FC | | | Edelman Financial Field | Ashburn, Virginia | 2012 |
| Huntsville City | | MLS Next Pro | Joe W. Davis Stadium | Huntsville | 2022 |
| Denton Diablos | | National Premier Soccer League | Mean Green Soccer Stadium | Denton, Texas | 2018 |
| Phoenix FC | | | Sun Devil Soccer Stadium | Phoenix | 2012 |
| VSI Tampa Bay | | | Plant City Stadium | Plant City | 2011 |
| Ventura County FC | | USL Championship | Dignity Health Sports Park | Los Angeles | 2014 |
| Colorado Rapids 2 | | MLS Next Pro | Denver Soccer Stadium | Denver | 2021 |
| Real Monarchs SLC | Jámison Olave | USL Championship | Rio Tinto Stadium | Sandy | 2014 |
| New York RB 2 | Ibrahim Sekagya | USL Championship | Columbia Soccer Stadium | New York | 2015 |
| Reno 1868 FC | Ian Russell | | Greater Nevada Field | Reno | 2015 |
| Capo FC | | NISA | Belson Stadium | San Juan Capistrano, California | 2006 |
| Irvine Zeta | | NISA | Highmark Stadium | Irvine, California | 2022 |
| Chicago Fire II | | MLS Next Pro | SeatGeek Stadium | Bridgeview | 2021 |
| Orlando City B | Marcelo Neveleff | | Titan Soccer Complex | Melbourne | 2015 |
| North Texas SC | Garbini Michel | USL League One | Toyota Stadium | Frisco | 2018 |
| New England Revolution II | | USL League One | Gillette Stadium | Boston | 2019 |
| Queensboro FC | | USL Championship | York College | New York City | 2019 |
| Fort Worth Vaqueros FC | | National Premier Soccer League | Farrington Field | Fort Worth, Texas | 2013 |
| Corpus Christi FC | | | Jack Dugan Family Soccer and Track Stadium | Corpus Christi | 2017 |
| Austin FC II | | MLS Next Pro | Parmer Field | Austin | 2022 |
